A whole blood microsampling assay for vancomycin: development, validation and application for pediatric clinical study.

TitleA whole blood microsampling assay for vancomycin: development, validation and application for pediatric clinical study.
Publication TypeJournal Article
Year of Publication2020
AuthorsMoorthy GS, Downes KJ, Vedar C, Zuppa AF
JournalBioanalysis
Volume12
Issue18
Pagination1295-1310
Date Published2020 Sep
ISSN1757-6199
Abstract

Vancomycin is a commonly used antibiotic, which requires therapeutic drug monitoring to ensure optimal treatment. Microsampling assays are attractive tools for pediatric clinical research and therapeutic drug monitoring. A LC-MS/MS method for the quantification of vancomycin in human whole blood employing volumetric absorptive microsampling (VAMS) devices (20 μl) was developed and validated. Vancomycin was stable in human whole blood VAMS under assay conditions. Stability for vancomycin was established for at least 160 days as dried microsamples at -78°C. This method is currently being utilized for the quantitation of vancomycin in whole blood VAMS for an ongoing pediatric clinical study and representative clinical data are reported.

DOI10.4155/bio-2020-0112
PubMed ID32945688
Grant ListK23HD091365, UL1TR001878 / GF / NIH HHS / United States