Pregnancy-associated changes in cervical noncoding RNA.

TitlePregnancy-associated changes in cervical noncoding RNA.
Publication TypeJournal Article
Year of Publication2020
AuthorsGerson KD, Haviland MJ, Neo D, Hecht JL, Baccarelli AA, Brennan KJm, Dereix AE, Ralston SJ, Hacker MR, Burris HH
JournalEpigenomics
Volume12
Issue12
Pagination1013-1025
Date Published2020 06
ISSN1750-192X
KeywordsAdult, Case-Control Studies, Cervix Uteri, Female, Gene Expression Profiling, Humans, MicroRNAs, Pregnancy, RNA, Long Noncoding
Abstract

To identify pregnancy-associated changes in cervical noncoding RNA (ncRNA), including miRNA and long noncoding RNA (lncRNA), and their potential effects on biologic processes. We enrolled 21 pregnant women with term deliveries (≥37 weeks' gestation) in a prospective cohort and collected cervical swabs before 28 weeks' gestation. We enrolled 21 nonpregnant controls. We analyzed miRNA, lncRNA and mRNA expression, applying a Bonferroni correction. Five miRNA and three lncRNA were significantly differentially (>twofold change) expressed. Putative miRNA targets are enriched in genes mediating organogenesis, glucocorticoid signaling, cell adhesion and ncRNA machinery. Differential cervical ncRNA expression occurs in the setting of pregnancy. Gene ontology classification reveals biological pathways through which miRNA may play a biologic role in normal pregnancy physiology.

DOI10.2217/epi-2019-0231
Alternate JournalEpigenomics
PubMed ID32808540
PubMed Central IDPMC7546170
Grant ListK23 ES022242 / ES / NIEHS NIH HHS / United States
P30 ES000002 / ES / NIEHS NIH HHS / United States
P30 ES009089 / ES / NIEHS NIH HHS / United States
UL1 TR001102 / TR / NCATS NIH HHS / United States