Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil.

TitleMultidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil.
Publication TypeJournal Article
Year of Publication2021
AuthorsViana ASlotfeldt, Botelho AMaria Nune, Moustafa AM, Boge CLK, Ferreira ALucia Pire, Carvalho MCícera da, Guimarães MAparecida, Costa Bde Souza S, de Mattos MCorrêa, Maciel SPires, Echevarria-Lima J, Narechania A, O'Brien K, Ryan C, Gerber JS, Carvalho BTeixeira F, Figueiredo AMarie Sá, Planet PJ
JournalEmerg Infect Dis
Volume27
Issue11
Pagination2825-2835
Date Published2021
ISSN1080-6059
Abstract

We typed 600 methicillin-resistant Staphylococcus aureus (MRSA) isolates collected in 51 hospitals in the Rio de Janeiro, Brazil, metropolitan area during 2014-2017. We found that multiple new clonal complex (CC) 5 sequence types had replaced previously dominant MRSA lineages in hospitals. Whole-genome analysis of 208 isolates revealed an emerging sublineage of multidrug-resistant MRSA, sequence type 105, staphylococcal cassette chromosome mec II, spa t002, which we designated the Rio de Janeiro (RdJ) clone. Using molecular clock analysis, we hypothesized that this lineage began to expand in the Rio de Janeiro metropolitan area in 2009. Multivariate analysis supported an association between bloodstream infections and the CC5 lineage that includes the RdJ clone. Compared with other closely related isolates, representative isolates of the RdJ clone more effectively evaded immune function related to monocytic cells, as evidenced by decreased phagocytosis rate and increased numbers of viable unphagocytosed (free) bacteria after in vitro exposure to monocytes.

DOI10.3201/eid2711.210097
Alternate JournalEmerg Infect Dis
PubMed ID34670645