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|Title||Identification of a novel intertypic recombinant species D human adenovirus in a pediatric stem cell transplant recipient.|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Kajon AE, Lamson D, Shudt M, Oikonomopoulou Z, Fisher BT, Klieger S, St George K, Hodinka RL|
|Journal||J Clin Virol|
|Date Published||2014 Dec|
|Keywords||Adenovirus Infections, Human, Adenoviruses, Human, Child, DNA, Viral, Feces, Female, Genome, Viral, Humans, Molecular Sequence Data, Plasma, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Stem Cell Transplantation, Transplant Recipients, Urine|
BACKGROUND: Human adenoviruses (HAdV) are known opportunistic pathogens in hematopoietic stem cell transplant (SCT) recipients. The detection of HAdV infection in children after SCT has been implicated as a determinant of poor outcome but specific associations between HAdV species or individual HAdV types and disease are poorly understood.
OBJECTIVES: Characterization of a HAdV-D strain isolated from multiple clinical specimens of an 11-year-old female recipient of a matched unrelated donor peripheral SCT for T-cell lymphoma and case report.
STUDY DESIGN: Archived HAdV PCR-positive plasma, urine, and stool specimens were processed for virus isolation and detailed molecular typing. Complete genomic sequencing was carried out on 2 isolates.
RESULTS: The patient tested positive for HAdV DNA by real-time PCR of a stool specimen at 44 days after initiation of a SCT conditioning regimen. In the subsequent 3 months, HAdV was detected in plasma, urine and stool specimens in association with symptoms of gastroenteritis and hemorrhagic cystitis. A novel HAdV-D with a HAdV20-like hexon gene was isolated from both urine and stool specimens. All isolates yielded identical restriction profiles with endonucleases BamHI, BglII, BstEII, HindIII, PstI and SmaI. Analysis of 2 complete genomic sequences further identified the virus as a novel intertypic recombinant HAdV-D (P20/H20/F42) closely related to HAdV42.
CONCLUSIONS: This case highlights the identification of a previously unknown HAdV-D from an immunocompromised host. In this patient, the course of adenovirus infection is compatible with reactivation of a latent virus or a primary opportunistic infection. Adenoviremia in this patient resolved without definitive adenovirus-directed antiviral therapy.
|Alternate Journal||J. Clin. Virol.|
|PubMed Central ID||PMC4276453|
|Grant List||HHSN2722011000040C / / PHS HHS / United States |
HHSN272201100040C / AI / NIAID NIH HHS / United States