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|Title||Correlation of Clinical Outcomes With Multiplex Molecular Testing of Stool From Children Admitted to Hospital With Gastroenteritis in Botswana.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Pernica JM, Steenhoff AP, Welch H, Mokomane M, Quaye I, Arscott-Mills T, Mazhani L, Lechiile K, Mahony J, Smieja M, Goldfarb DM|
|Journal||J Pediatric Infect Dis Soc|
|Date Published||2016 Sep|
|Keywords||Anti-Bacterial Agents, Botswana, Child, Preschool, Diarrhea, Infantile, Feces, Female, Gastroenteritis, Hospitalization, Humans, Infant, Male, Malnutrition, Multiplex Polymerase Chain Reaction, Prospective Studies, Treatment Outcome|
BACKGROUND: Diarrheal disease is a leading cause of death for young children. Most pediatric gastroenteritis is caused by viral pathogens; consequently, current recommendations advocate against routine antibacterial therapy if children present without bloody stools.
METHODS: In this prospective cohort study, we enrolled children with severe acute gastroenteritis admitted to hospital in Botswana. Details of presenting history, physical examination, and course in the hospital were recorded. Stool samples were characterized using a 15 pathogen polymerase chain reaction assay.
RESULTS: There were 671 participants with a median age of 8.3 months; 77 (11%) had severe acute malnutrition. Only 74 children had bloody stools, of whom 48 (65%) had a detectable bacterial pathogen, compared to 195 of 592 (33%) of those without. There were 26 deaths (3.9%). Covariates associated with death in multivariable logistic regression were the detection of any of Campylobacter/Shigella/enterotoxigenic Escherichia coli (odds ratio [OR] 2.57, 95% confidence interval [CI] 1.07-6.17), severe acute malnutrition (OR 4.34, 95% CI 1.79-10.5), and antibiotic therapy (OR 8.82, 95% CI 2.03-38.2). There was no significant association between bloody stools and death, and the effect of Campylobacter/Shigella/enterotoxigenic E. coli infection on death was not modified by the presence of bloody stools.
CONCLUSIONS: Detection of bacterial enteropathogens is associated with increased mortality in children in sub-Saharan Africa. Unfortunately, most children with these infections do not have bloody stools, and bloody dysentery was not found to be associated with worse outcomes. Clinical trials evaluating outcomes associated with more aggressive diagnostic strategies in children presenting with severe acute gastroenteritis in sub-Saharan Africa should be undertaken.
|Alternate Journal||J Pediatric Infect Dis Soc|
|PubMed Central ID||PMC5125452|
|Grant List||P30 AI045008 / AI / NIAID NIH HHS / United States|