Bacterial and cytokine mixtures predict the length of gestation and are associated with miRNA expression in the cervix.

TitleBacterial and cytokine mixtures predict the length of gestation and are associated with miRNA expression in the cervix.
Publication TypeJournal Article
Year of Publication2017
AuthorsSanders AP, Gennings C, Svensson K, Motta V, Mercado-Garcia A, Solano M, Baccarelli AA, Tellez-Rojo MM, Wright RO, Burris HH
JournalEpigenomics
Volume9
Issue1
Pagination33-45
Date Published2017 01
ISSN1750-192X
KeywordsAdolescent, Adult, Biomarkers, Cervix Uteri, Cytokines, Female, Humans, MicroRNAs, Pregnancy, Pregnancy Complications, Infectious, Premature Birth, RNA, Messenger, Vaginosis, Bacterial
Abstract

AIM: Bacterial vaginosis may lead to preterm birth through epigenetic programming of the inflammatory response, specifically via miRNA expression.

METHODS: We quantified bacterial 16S rRNA, cytokine mRNA and 800 miRNA from cervical swabs obtained from 80 women at 16-19 weeks' gestation. We generated bacterial and cytokine indices using weighted quantile sum regression and examined associations with miRNA and gestational age at delivery.

RESULTS & DISCUSSION: Each decile of the bacterial and cytokine indices was associated with shorter gestations (p < 0.005). The bacterial index was associated with miR-494, 371a, 4286, 185, 320e, 888 and 23a (p < 0.05). miR-494 remained significant after false discovery rate correction (q < 0.1). The cytokine index was associated with 27 miRNAs (p < 0.05; q < 0.01).

CONCLUSION: Future investigation into the role of bacterial vaginosis- and inflammation-associated miRNA and preterm birth is warranted.

DOI10.2217/epi-2016-0095
Alternate JournalEpigenomics
PubMed ID27936911
PubMed Central IDPMC5514975
Grant ListR01 ES021357 / ES / NIEHS NIH HHS / United States
P30 ES000002 / ES / NIEHS NIH HHS / United States
R01 ES013744 / ES / NIEHS NIH HHS / United States
P30 ES023515 / ES / NIEHS NIH HHS / United States
R01 ES014930 / ES / NIEHS NIH HHS / United States
R01 ES020268 / ES / NIEHS NIH HHS / United States
K23 ES022242 / ES / NIEHS NIH HHS / United States
P30 ES009089 / ES / NIEHS NIH HHS / United States