Age and fecal microbial strain-specific differences in patients with spondyloarthritis.

TitleAge and fecal microbial strain-specific differences in patients with spondyloarthritis.
Publication TypeJournal Article
Year of Publication2018
AuthorsStoll ML, Weiss PF, Weiss JE, Nigrovic PA, Edelheit BS, S Bridges L, Danila MI, Spencer CH, Punaro MG, Schikler K, Reiff A, Kumar R, Cron RQ, Morrow CD, Lefkowitz EJ
JournalArthritis Res Ther
Volume20
Issue1
Pagination14
Date Published2018 Jan 30
ISSN1478-6362
Abstract

BACKGROUND: Prior studies have demonstrated abnormalities in the composition of the gastrointestinal microbiota in pediatric and adult patients with spondyloarthritis (SpA). In particular, diminished fecal abundance of Faecalibacterium prausnitzii and abnormalities in both directions in the abundance of the Bacteroides genus have been identified.

METHODS: We obtained fecal specimens from 30 children with treatment-naïve enthesitis-related arthritis (ERA) and 19 healthy controls, as well as specimens from 11 adult patients with longstanding SpA and 10 adult healthy controls. All of the samples underwent sequencing of the 16S ribosomal DNA. A subset of the pediatric fecal samples was subjected to shotgun metagenomics sequencing.

RESULTS: ERA patients had decreased abundance of the anti-inflammatory F. prausnitzii A2-165 strain (41 ± 28% versus 54 ± 20% of all sequences matching F. prausnitzii, p = 0.084) and an increased abundance of the control F. prausnitzii L2/6 strain (28 ± 28% versus 15 ± 15%, p = 0.038). Similar trends were observed in adults with longstanding SpA (n = 11) and controls (n = 10). In contrast, the fecal abundance of Bacteroides fragilis was increased in ERA subjects (2.0 ± 4.0% versus 0.45 ± 0.7% of all sequences, p = 0.045), yet was diminished in adult subjects (0.2 ± % versus 1.0 ± % of all sequences, p = 0.106). Shotgun metagenomics sequencing of the fecal DNA in the pediatric subjects revealed diminished coverage of the butanoate pathway (abundance normalized to controls of 1 ± 0.48 versus 0.72 ± 0.33 in ERA, p = 0.037).

CONCLUSIONS: The anti-inflammatory F. prausnitzii A2-165 strain appears to be depleted in both pediatric and adult SpA. In contrast, B. fragilis may be depleted in adult disease yet abundant in pediatric SpA, suggesting developmental effects on the immune system.

DOI10.1186/s13075-018-1510-6
PubMed ID29382366
Grant ListP60 AR064172 / / National Institute of Arthritis and Musculoskeletal and Skin Diseases / United States
K23 AR062100 / / National Institute of Arthritis and Musculoskeletal and Skin Diseases / United States
P30 CA013148 / / Comprehensive Cancer Center, University of Alabama at Birmingham / United States
5P30AI027767 / / Center for AIDS Research, UAB / United States
UL1TR001417 / / Center for Clinical and Translational Science, University of Illinois at Chicago (US) / United States